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(一) 在經徹底冲洗的兔骨骼肌製劑中,[L-α]甘油磷酸和琥珀酸的氧化彼此干涉。琥珀酸對[L-α]甘油磷酸氧化的抑制作用能因加入抑制琥珀酸脫氫酶的焦磷酸而解除。 (二) 當用細胞色素c作受體時[L-α]甘油磷酸,還原輔酶I和琥珀酸三者同時氧化時總氧化速度僅相當其中氧化速度最高者即還原輔酶I單獨氧化的速度。[L-α]甘油磷酸氧化酶系也因[2,3]二氫硫基丙醇的處理而失效。 (三) 當用[2,6]二氯酚靛酚作受體時[L-α]甘油磷酸和琥珀酸同時氧化時速度完全等於二底料單獨氧化時速度的和。[L-α]甘油磷酸的氧化不受苯代氨甲酸乙酯的影響。 (四) 本文結果說明[L-α]甘油磷酸的氧化不通過細胞色素b而通過中間因子和細胞色素c連接。
(A) In a thoroughly rinsed rabbit skeletal muscle preparation, the oxidation of [L-α] glycerophosphate and succinate interfere with each other. The inhibitory effect of succinic acid on [L-α] glycerophosphate oxidation can be released by adding pyrophosphate which inhibits succinate dehydrogenase. (2) The total oxidation rate of [L-α] glycerophosphate, reduced coenzyme I and succinic acid simultaneously oxidized by cytochrome c only corresponded to the rate at which the highest oxidation rate, that is, reduced coenzyme I alone, was oxidized. [L-α] Glycerophosphate oxidase system also failed due to the treatment of [2,3] dihydrothiopropanol. (C) The rate of [L-α] simultaneous oxidation of glycerophosphate and succinate when [2,6] dichlorophenolindophenol is used as a receptor is exactly equal to the sum of the rates of the two substrates when they are oxidized separately. [L-α] Oxidation of glycerophosphate is unaffected by ethyl benzenecarbamate. (D) The results of this paper demonstrate that the oxidation of [L-α] glycerophosphate does not connect to cytochrome c through the intermediary of cytochrome b.