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用循环伏安法研究了亚甲蓝在活化的碳纤维微电极上电聚合成膜的方法和条件 ,并对该聚合膜修饰电极的电化学性质进行了探讨 .该膜修饰的电极对多巴胺有较强的催化作用 ,催化峰电流与底物浓度在 2 .0× 1 0 - 6～ 1 .2× 1 0 - 4 mol/ L范围内呈线性关系 .在一定的条件下 ,该膜修饰电极能有效消除多巴胺和抗坏血酸的相互干扰 ,使多巴胺和抗坏血酸的催化峰电位差达 2 1 0 m V.用这种微电极对注射液中的多巴胺进行测定 ,回收率在 98.75 %～1 0 1 .5 %之间 .该检测过程无需除氧 ,可望用于活体分析 .
The cyclic voltammetry was used to study the method and conditions for the electropolymerization of methylene blue on activated carbon fiber microelectrodes and the electrochemical properties of the polymer modified electrodes were also discussed. Strong catalytic activity and catalytic peak current were linear with substrate concentration in the range of 2.0 × 10 -6 ~ 1.2 × 10 4 mol / L. Under certain conditions, Effectively eliminate the mutual interference of dopamine and ascorbic acid, so that the catalytic peak potential difference between dopamine and ascorbic acid up to 210 m. With this microelectrode in the determination of dopamine injection, the recovery was 98.75% ~ 1 0 1 .5 %. The test process without oxygen, is expected to be used for in vivo analysis.