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目的探讨中药补骨脂素和女贞子对小鼠B16黑素瘤细胞c-KIT表达的影响及意义。方法体外培养小鼠B16黑素瘤细胞,用反转录-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western blot)测定补骨脂素和女贞子对小鼠B16黑素瘤细胞c-KIT表达的影响,用Transwell小室法检测补骨脂素和女贞子对小鼠B16黑素瘤细胞迁移功能的影响。结果补骨脂素与女贞子用药组迁移到膜下层的每视野细胞个数分别较对照组多(P<0.05),并呈剂量依赖关系;用药组c-KIT m RNA、c-KIT蛋白表达分别比对照组增加,差异均有统计学意义(P<0.05)。结论补骨脂素和女贞子可能是通过促进小鼠B16黑素瘤细胞c-KIT的表达而促进其迁移功能,从而对白癜风产生治疗作用。
Objective To investigate the effect and significance of psoralen and Ligustrum lucidum on the expression of c-KIT in mouse B16 melanoma cells. Methods Murine B16 melanoma cells were cultured in vitro. The expression of psoralens and Ligustrum lucidum in mice B16 melanoma cells were determined by reverse transcription - polymerase chain reaction (RT - PCR) and Western blot. c-KIT expression. The effect of psoralen and Ligustrum lucidum on the migration of mouse B16 melanoma cells was assayed by Transwell chamber assay. Results The number of cells per visual field migrated to the subfractor of the psoralen and Ligustrum lucidum groups was more than that of the control group (P <0.05), and the dose-dependent manner. The c-KIT m RNA and c-KIT protein Compared with the control group, the expression increased significantly (P <0.05). Conclusion Psoralens and Ligustrum lucidum may promote the migratory function of c-KIT in mouse B16 melanoma cells, which may have a therapeutic effect on vitiligo.