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目的:建立和评价海人酸杏仁核微量注射点燃癫痫动物模型。方法:选用雄性Wistar大鼠,以杏仁核外侧基底核为给药靶点,在立体定位仪下,微量注射海人酸1μg后进行大鼠的行为学和电生理学观察,并分别于第6、12、72 h和7、14、21 d对大鼠脑组织进行病理学观察。结果:海人酸微量注射后实验大鼠出现典型的癫痫发作过程,顶叶皮层脑电图依次出现多种形式的痫性放电,HE染色显示海马和颞叶皮层神经细胞的相应病理变化,点燃成功率为90%。结论:采用杏仁核海人酸微量注射方法成功建立了Wistar大鼠点燃癫痫模型,该模型适用于颞叶癫痫的相关研究。
OBJECTIVE: To establish and evaluate the microinjection of kainate almond into the animal model of epilepsy. Methods: The male Wistar rats were used to target the basolateral nucleus of amygdala. Under the stereotaxic instrument, the rats were subjected to behavioral and electrophysiological observation after microinjection of 1μg of kainic acid, The pathological changes of rat brain were observed at 12, 72 h and 7, 14 and 21 days. Results: The typical epileptic seizures occurred in the experimental rats after microinjection of kainate into the brain, and the epileptic seizures occurred in the parietal cortex electroencephalogram. HE staining showed the corresponding pathological changes of neurons in the hippocampus and temporal cortex, The success rate is 90%. Conclusion: The epilepsy model of Wistar rats was successfully established by microinjection of amygdala into the kainic acid solution. This model is suitable for the study of temporal lobe epilepsy.