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本文叙述了莫桑比克罗非鱼的精液在以5％的甲醇和15％的奶粉作低温防护剂时的冷冻贮存方法。用此法冷冻精子解冻后精子的运动能力大约是以5％或10％的二甲亚砜(DMSO)代替甲醇作低温防护剂时的二倍。本文还详细描述了低温防护剂的最适浓度,冷却和解冻加温的最适速度以及稀释度和平衡时间对精子的影响。还报导了受精能力的试验:以冷冻贮存的精子受精后的胚胎有64.3％±34.2％可以正常发育到胚孔封闭期而未经冷冻的对照组为57.5±10.5％。
This article describes the frozen storage of semen from tilapia in Mozambique when it is cryogenically protected with 5% methanol and 15% milk powder. This method of sperm freezing thaw sperm motility is about 5% or 10% of dimethyl sulfoxide (DMSO) instead of methanol as a cryogenic protective agent twice. This article also describes in detail the optimum concentration of cryoprotectant, the optimum temperature for cooling and thawing, and the effect of dilution and equilibration time on sperm. A fertilization test was also reported: 64.3% ± 34.2% of frozen-stored spermatozoa fertilized embryos developed normally to the embryo confinement compared to 57.5 ± 10.5% in the control group without freezing.