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目的 :观察急性低氧和间断低氧习服对大鼠主动脉收缩和舒张功能的影响。方法 :用去甲肾上腺素 (NE)诱发大鼠离体主动脉环的收缩 ,反映收缩功能的变化 ,主动脉环以 0 .6 2 μmol/LNE预收缩后用乙酰胆碱 (Ach)舒张对抗 ,反映舒张功能的变化。结果 :与常氧对照组相比 ,急性低氧和间断低氧习服大鼠主动脉对去甲肾上腺素 (NE1 0 -9,1 0 -8,1 0 -7mol/L)介导的收缩反应显著增强 (P <0 .0 5～ 0 .0 1 ) ,而对Ach各浓度 (1 0 -9～ 1 0 -5mol/L)诱导的内皮依赖性舒张反应显著减弱 (P <0 .0 1 )。结论 :急性低氧和间断低氧习服后 ,大鼠主动脉对缩血管药物的收缩反应增强 ,内皮依赖性的舒张反应减弱 ,内皮受到了一定程度的损伤。
Objective: To observe the effects of acute hypoxia and intermittent hypoxic acclimatization on contractility and diastolic function of aorta in rats. Methods: The contractions of isolated aortic rings were induced by norepinephrine (NE) in rats. The aortic rings were pre-contracted with 0.62 μmol / LNE and then contracted with acetylcholine (Ach) Changes in diastolic function. RESULTS: Compared with normoxia control group, noradrenalin (NE1 0 -9,1 0 -8,1 0 -7 mol / L) mediated contraction of the aorta in acute hypoxic and intermittent hypoxic rats (P <0.05-0.01), while the endothelium-dependent vasorelaxation induced by various concentrations of Ach (10-9 ~ 10-5mol / L) was significantly attenuated (P <0. 0 1 ). CONCLUSION: After acute hypoxia and intermittent hypoxia, the contractile responses of vasoconstrictors to the aorta of rats are enhanced, endothelium-dependent vasodilatation is weakened, and the endothelium is damaged to a certain extent.