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目的 研究乌灵胶囊改善阿尔茨海默病模型小鼠(5×FAD)认知功能及缝隙连接蛋白43(Cx43)的作用机制.方法 用随机区组法将5×FAD小鼠随机分为模型组(n=10)和实验组(n=10),同时选择健康小鼠作为正常组(n=10).在小鼠4月龄时,实验组将乌灵胶囊按150 mg· kg -1· d-1的用药剂量于0.5%CMC-Na溶液中定容至40 mL,于每日上午9时灌胃给药1次,持续给药2个月.正常组与模型组小鼠接受常规膳食.用水迷宫和Y形迷宫实验评价小鼠的认知功能.用免疫组化法检测小鼠脑内Cx43蛋白的表达情况.结果给药后训练第3天,实验组与模型组小鼠逃避潜伏期分别为(58.0 ±1.3 ), (63.0 ±1.2)s,组间比较差异有统计学意义(P＜0.05);实验组小鼠平台所在象限停留时间(32.0 ±1.5)s及跨平台次数(4.6 ±1.1)s均显著高于模型组的(19.0 ±1.7),( 1.9 ±1.2) s,差异均有统计学意义(均P＜0.05);实验组小鼠2个月后对被动刺激安全逃避的错误反应次数为(3.9 ±0.5)次,明显低于模型组小鼠的(6.3 ±1.6)s,差异有统计学意义(P＜0.05).实验组小鼠脑内Cx43蛋白的表达明显高于模型组小鼠,差异有统计学意义( P＜0.05).结论 乌灵胶囊可通过增加Cx43蛋白的分泌改善认知功能.“,”Objective To investigate the effect of Wuling Capsule on cognitive function of Alzheimer disease model mice (5×FAD) and the mechanism of connexin 43 ( Cx43).Methods The 5xFAD mice were randomly divided into model group ( n =10 ) and experimental group (n=10),healthy mice as normal group (n=10).At 4 months old,the mice in the experimental group were given Wuling Capsule by gastrointesti-nal administration once a day at 9:00 am at a dose of 150 mg· kg-1· d-1 for 2 months, which was weighed to 40 mL in a 0.5% CMC-Na solu-tion.The normal group and model group received regular diet .The cog-nitive function of mice was evaluated by Water maze and Y maze .Immu-nohistochemical method was used to detect the expression of Cx 43 protein in the brain of mice.Results After the administration , the escape la-tency of the experimental group and model group on the third day after training was (58.0 ±1.3), (63.0 ±1.2) s, and the difference was sta-tistically significant ( P ＜0.05 ) . The quadrant retention time (32.0 ±1.5)s and cross-platform times (4.6 ±1.1) s of the experi-mental mouse platform were significantly higher than the model group (19.0 ±1.7), (1.9 ±1.2 ) s ( all P ＜0.05 ) .After two months of administration, the number of erroneous responses (3.9 ±0.5) times in the experimental group to passive stimuli for safe escape was significantly lower than that in the model group (6.3 ±1.6 ) times ( P＜0.05) .Conclusion Wuling Capsule can improve the cognitive function by increasing the secretion of Cx43 protein.