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目的:探讨新疆维吾尔族白细胞介素-13基因单核苷酸多态性与慢性阻塞性肺疾病易感性的关系。方法:140例慢性阻塞性肺疾病稳定期患者和140例健康者,应用PCR、DNA测序法检测白细胞介素-13基因启动子-1055C/T位点基因型分布情况;用ELISA测定血清中白细胞介素-13水平。结果:2组白细胞介素-13基因启动-子1055 C/T位点各基因型频率和等位基因频率的分布差异均有统计学意义(P均<0.01);各基因型携带者血浆白细胞介素-13水平差异有统计学意义(P<0.05)。结论:白细胞介素-13基因启动子-1055C/T位点的单核苷酸多态性与新疆和田地区维吾尔族人群的慢性阻塞性肺疾病易感性存在相关性。
Objective: To investigate the relationship between polymorphism of Uygur IL-13 gene and the susceptibility to chronic obstructive pulmonary disease in Xinjiang Uygur Autonomous Region. Methods: One hundred and forty patients with stable chronic obstructive pulmonary disease (COPD) and 140 healthy controls were enrolled in this study. Genotype distribution of -1055C / T locus in promoter of IL-13 gene was detected by PCR and DNA sequencing. Serum leukocyte Interleukin-13 levels. Results: There were significant differences in the frequencies of genotypes and alleles in promoter - 1055 C / T locus between the two groups (all P <0.01). The levels of leukocyte The level of IL-13 was significantly different (P <0.05). Conclusion: The single nucleotide polymorphism at -1055C / T locus of interleukin-13 gene promoter is associated with the susceptibility to chronic obstructive pulmonary disease in Uygur population in Hetian area.