论文部分内容阅读
以小剂量大肠杆菌内毒素分次注射复制了家兔病理性氧供依赖性(POSD)模型。采用再呼吸法造成渐进性低氧以降低总氧运输量(To_2),从而考察氧耗量(Vo_2)随To_2降低而变化的规律。结果表明,在渐进性低氧过程,动、静脉血氧含量(Cao_2,Cvo_2)的下降趋势达显著水平(P<0.01,ANOVA),但内毒素组Cvo_2下降较为缓慢,故其动静脉血氧含量差(Cao_2-Cvo_2)越来越小,对照组则相反。在To_2-Vo_2的关系中,对照组可清楚地分为“非依赖”与“依赖”两部分,其总氧运输量临界值和临界氧提取率分别为10.18±1.69ml·min~(-1)·kg~(-1)和0.85±0.10;内毒素组从未出现坪区,表明从呼吸空气开始Vo_2对To_2即呈依赖关系。对照组依赖段的斜率,即氧提取率为0.730,内毒素组斜率为0.473,两者差别达显著水平(P<0.05)。本实验成功地复制出兔POSD模型及有效的研究途径。
Rabbit pathological oxygen-dependent (POSD) model was replicated by small-dose injection of E. coli endotoxin. Respiration method was used to induce gradual hypoxia to reduce the total oxygen transport (To_2), thus investigating the variation of oxygen consumption (Vo_2) with decreasing To_2. The results showed that during the process of gradual hypoxia, the decline tendency of arterial and venous oxygen content (Cao_2, Cvo_2) reached significant level (P <0.01, ANOVA), but the decrease of Cvo_2 in endotoxin group was relatively slow. Therefore, arteriovenous oxygen The content difference (Cao_2-Cvo_2) is getting smaller and smaller, while the control group is the opposite. In the relationship of To_2-Vo_2, the control group can be clearly divided into two parts, “independent” and “dependent”, the total oxygen transport and critical oxygen extraction rates were 10.18 ± 1.69ml · min ~ (-1) ) · Kg -1 and 0.85 ± 0.10 respectively. There was no plateau in endotoxin group, which indicated that Vo 2 was dependent on To_2 from the beginning of respiratory air. The slope of the dependent segment of the control group, namely, the rate of oxygen extraction was 0.730 and the slope of the endotoxin group was 0.473, with a significant difference (P <0.05). The experiment successfully replicated rabbit POSD model and effective research approach.