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采用ICCD光谱探测系统对不同浓度番茄红素的二硫化碳溶液吸收谱的相对吸收强度进行了测量,结果显示在一定浓度范围内,番茄红素稀溶液的吸收规律满足朗伯-比尔定律;分别用丙酮、正己烷、石油醚、苯、乙酸乙酯和二硫化碳作为溶剂对番茄红素可见吸收光谱进行了测量,对结果进行分析后发现苯、乙酸乙酯和二硫化碳的番茄红素溶液的特征吸收峰的波长位置与以丙酮作为溶剂相比有不同程度的红移效应;番茄红素-丙酮溶液中加入水后溶液颜色随着加水量的增加逐渐变浅,溶液吸光度降低,当丙酮与水的体积比为4∶1时吸收光谱在紫外出现一新的吸收峰。产生这些现象的原因是番茄红素溶于不同溶剂时,溶剂分子对番茄红素分子作用不同。用荧光光度计采集不同浓度的番茄红素丙酮溶液的荧光光谱,结果表明番茄红素溶液的荧光光谱主要集中在500~680 nm波段,浓度低于50μg.mL-1时,番茄红素的荧光强度随着浓度的增加而呈线性增加。当浓度高于60μg.mL-1时,荧光强度因为番茄红素分子间的相互作用而下降。
The relative absorption intensity of the absorption spectra of different concentration of lycopene with carbon disulfide solution was measured by ICCD spectroscopy system. The results showed that the absorbance law of dilute solution of lycopene satisfied Lambert-Beer’s law at a certain concentration range, , N-hexane, petroleum ether, benzene, ethyl acetate and carbon disulfide were used as solvents to measure the apparent absorption spectra of lycopene. The results were analyzed and found that the characteristic absorption peaks of the lycopene solution of benzene, ethyl acetate and carbon disulfide Wavelength position and acetone as a solvent compared to different degrees of redshift effect; lycopene - acetone solution after adding water, the color of the solution with the increase of water gradually become lighter, the solution absorbance decreases, when the volume ratio of acetone and water Absorption spectrum at 4: 1 shows a new absorption peak in UV. The reason for these phenomena is that when lycopene is dissolved in different solvents, the effect of solvent molecules on lycopene molecules is different. The fluorescence spectra of different concentrations of lycopene acetone solution were collected by fluorophotometer. The results showed that the fluorescence spectra of lycopene solution mainly concentrated in the wavelength range of 500-680 nm. When the concentration was lower than 50μg.mL-1, the fluorescence of lycopene The intensity increases linearly with increasing concentration. When the concentration is higher than 60μg.mL-1, the fluorescence intensity decreases due to the interaction between lycopene molecules.