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为了探讨生后大鼠脑室下层神经元前体细胞离体培养时的神经活性物质特征和形态学特征 ,本研究以细胞特异性的抗体进行免疫细胞化学反应并测量了这些细胞的形态学指标胞体平均直径和胞体椭圆率 (胞体最小径与最大径的比值 )。取脑室下层细胞接种于覆有多聚赖氨酸的 2 4孔培养板 ,培养液为 Neurobasal Medium( Gibco) /B2 7,培养条件为 5 % CO2 ,3 7℃。结果如下 :在培养 1d时 ,绝大多数 (大于 90 % )脑室下层源细胞呈 β-3型微管蛋白阳性 ,且这些细胞也都表达多唾液酸神经粘连分子。此时 ,大多数β-3型微管蛋白阳性细胞胞体呈椭圆形 (胞体平均直径为 8.42± 1.0 3μm;胞体椭圆率为 0 .5 7± 0 .12 ) ,且在其两极各长出一短的、无分支的突起 ;并有近 2 0 %者为球形无突起细胞 (胞体平均直径为 7.2 0± 1.0 4μm) ,且它们大多呈细胞与细胞相连接状态。随着培养时间的延长 ,这些细胞亦呈上述椭圆形有突起样细胞变化 ,但仍可见它们呈串珠样连接。培养至第 3～5 d,这些脑室下层源细胞的体积增大 (胞体平均直径为 9.0 7± 1.0 7μm)、突起较前增长但仍几无分支。免疫细胞化学反应显示 ,此时培养中的脑室下层源细胞仍大多呈β-3型微管蛋白阳性和多唾液酸神经粘连分子阳性。至培养的第 7d,这些β-3型微管蛋白阳性
In order to investigate the characteristics and morphological features of neuroactive substances of rat subventricular zone neuron precursor cells cultured in vitro, cell-specific antibody was used in immunocytochemistry to measure the morphology of these cells Mean diameter and ellipticity of cell body (the ratio of the smallest to the largest diameter of the cell body). The cells in the inferior ventricle were seeded on a 24-well plate coated with polylysine. The culture medium was Neurobasal Medium (Gibco) / B2 7 and the culture conditions were 5% CO2 and 37 ° C. The results were as follows: At day 1 of culture, the vast majority (more than 90%) of the cells in the subventricular zone were positive for β-3 tubulin, and these cells also expressed polysialic adhesion molecules. At this time, the majority of β-3 tubulin positive cell bodies were oval (cell body average diameter 8.42 ± 1.0 3μm; cell body ellipticity was 0. 57 ± 0.12), and in each of its poles grow a Short and unbranched protrusions. Nearly 20% of them were spherical and non-protuberant cells (mean diameter of cell body was 7.2 ± 1.04μm), and most of them were connected with cells. As the incubation time extended, these cells also showed the above-mentioned elliptical prominocytic changes, but they were still seen as beaded connections. After 3 to 5 days of culture, the volume of the source cells in the inferior ventricle increased (the average size of the cell body was 9.07 ± 1.07μm), and the protrusions grew more but did not branch. Immunocytochemistry showed that most of the cells in the subventricular zone in culture were still positive for β-3 tubulointerstitial and polysialin adhesion molecules. At day 7 of culture, these β-3 tubules were positive