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用磷脂脂肪酸法(PLFA)分析广东鹤山两种人工林——针叶林和荷木林不同土层(0～20 cm、20～40 cm、40～60 cm的)土壤微生物群落结构。结果表明,两种人工林的磷脂脂肪酸总量(PLFAs总量)、细菌特征脂肪酸(细菌PLFAs)、真菌特征脂肪酸(真菌PLFAs),以及革兰氏阳性菌和革兰氏阴性菌特征脂肪酸的含量都是0～20 cm土层最高;荷木林的PLFAs总量高于针叶林,但这种差异随土壤深度增加而递减,且与土壤有机碳、全氮含量呈正相关。在相同土层,细菌PLFAs含量均显著高于真菌PLFAs含量。主成分分析表明,土壤微生物多样性及其丰富度受林型和土层综合作用的影响;单一的PLFAs中,i13:0、a13:0、17:0、cy17:0和16:0等脂肪酸对第一主成分的贡献较大;i16:0、cy19:0、18:1ω9 c、i15:0、18:2ω6 c和a17:0等脂肪酸对第二主成分贡献较大。
Phospholipid fatty acid assay (PLFA) was used to analyze the soil microbial community structure in two different plantations, coniferous forest and holmwood (0-20 cm, 20-40 cm, 40-60 cm) in Heshan, Guangdong Province. The results showed that the contents of total phospholipid fatty acids (total PLFAs), bacterial characteristic fatty acids (bacterial PLFAs), fungal fatty acids (fungi PLFAs), and characteristic fatty acids of Gram-positive and Gram-negative bacteria The soil layer of 0-20 cm soil layer was the highest. The total PLFAs of the woodland were higher than that of the coniferous forest, but the difference decreased with soil depth increasing, and positively correlated with soil organic carbon and total nitrogen. In the same soil layer, the content of bacterial PLFAs was significantly higher than that of fungi PLFAs. Principal component analysis showed that the diversity and abundance of soil microorganisms were affected by the combined effects of forest types and soil layers. In single PLFAs, fatty acids such as i13: 0, a13: 0, 17: 0, cy17: 0 and 16: 0 The contribution of the first principal component is larger; fatty acids such as i16: 0, cy19: 0,18: 1ω9c, i15: 0,18: 2ω6c and a17: 0 contribute more to the second principal component.