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目的:探索2,4-二羟基二苯甲酮对CCl4所致急性肝损伤的保护作用。方法:采用CCl4致小鼠急性肝损伤模型,染毒24 h后,测定血清中丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)和乳酸脱氢酶(lactate dehydrogenase,LDH)的活性;留取肝脏组织,常规石蜡包埋切片,HE染色,光镜观察肝脏组织病理变化;制备肝匀浆,测定肝中还原型谷胱甘肽(reducedglutathione hormone,GSH)、氧化型谷胱甘肽(oxidized glutathione,GSSG)和丙二醛(malondialdehyde,MDA)的含量。结果:与对照组比较,模型组小鼠血清中ALT,AST和LDH活性升高,肝组织中GSH/GSSG比值下降,MDA含量增加,肝小叶中心出现大量坏死细胞;2,4-二羟基二苯甲酮各剂量组血清ALT,AST和LDH水平明显降低,使肝组织中GSH/GSSG比值升高,MDA含量下降,肝小叶中心变性坏死细胞减少,坏死区域缩小。结论:2,4-二羟基二苯甲酮对CCl4引起的小鼠急性肝损伤有明显的保护作用。
Objective: To explore the protective effect of 2,4-dihydroxybenzophenone on acute liver injury induced by CCl4. Methods: Acute liver injury induced by CCl4 was induced in mice. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate The activity of lactate dehydrogenase (LDH) was removed. The liver tissues were collected and paraffin embedded sections were obtained. HE staining and light microscopy were used to observe the histopathological changes in the liver. Liver homogenates were prepared for determination of reduced glutathione hormone , GSH), oxidized glutathione (GSSG) and malondialdehyde (MDA) were measured. Results: Compared with the control group, the activity of ALT, AST and LDH in the model group was increased, the ratio of GSH / GSSG in the liver tissue was decreased, the content of MDA was increased and a large number of necrotic cells appeared in the center of hepatic lobule. The levels of serum ALT, AST and LDH in each dose of benzophenone significantly decreased, the ratio of GSH / GSSG in liver tissue increased, the content of MDA decreased, the number of denatured and necrotic cells in hepatic lobule center decreased and the necrotic area decreased. CONCLUSION: 2,4-Dihydroxybenzophenone has a significant protective effect on CCl4-induced acute liver injury in mice.