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目的确定长沙地区引起急性胃肠炎诺如病毒的类型。方法从128例腹泻患者粪便中,提取RNA。将RNA进行逆转录、扩增、测序,并通过构建基因进化树进行分析导致长沙地区患者感染的诺如病毒的主要种类。结果63个样品中含有诺如病毒,分别为GII-3、GII-4、GII-12三种类型,其中GII-4为主要病原体。结论在长沙地区,诺如病毒GII-4是引起病毒性胃肠炎的的主要致病株。GII-4基因进化快速,已成为全球范围内导致急性胃肠炎的最常见病毒株。
Objective To determine the type of Norovirus that causes acute gastroenteritis in Changsha. Methods RNA was extracted from the urine of 128 diarrhea patients. RNA reverse transcribed, amplified, sequenced and analyzed by constructing a phylogenetic tree led to the main species of Norovirus infected in Changsha patients. Results Sixty-three samples contained norovirus, which were GII-3, GII-4 and GII-12, of which GII-4 was the main pathogen. Conclusions In Changsha, norovirus GII-4 is the main pathogenic strain that causes viral gastroenteritis. The rapidly evolving GII-4 gene has become the most common viral strain that causes acute gastroenteritis worldwide.