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在233例多种肿瘤的石蜡切片和细胞学涂片上进行银染核仁形成区(AgNoRs)染色,并取各种正常组织作对照,其染色结果和定量观察表明:该法有助于临床病理学鉴别诊断,是肿瘤病理学研究的有效方法。本文从方法学的角度探讨AgNoRs染色法的可行性及其临床意义。对应用该法时可能碰到的有关问题进行了实验对比,并提出了解决途径。
Silver staining nucleolar forming area (AgNoRs) was stained on paraffin sections and cytology slides of 233 cases of various tumors, and various normal tissues were used as controls. The staining results and quantitative observations indicate that this method is helpful for clinical Differential diagnosis of pathology is an effective method for the study of tumor pathology. This article discusses the feasibility and clinical significance of AgNoRs staining method from the perspective of methodology. Experiments were conducted to compare the problems that may be encountered when applying the method, and solutions were proposed.