应用RT—PCR技术，以神经内分泌蛋白基因产物9．5（PGP9．5）为肿瘤标志物，评价了这一检测系统的效果。琼脂糖凝胶电泳分析RT－PCR产物发现神经母细胞瘤细胞株均为阳性，正常人外周血阴性，其敏感度达1个瘤细胞／107外周血多形核细胞。临床上测定39例患儿外周血、骨髓及组织标本证实RT－PCR检测PGP9．5为一敏感特异的测定方法。 Using RT-PCR technology, the neuroendocrine protein gene product 9.5 (PGP9.5) was used as a tumor marker to evaluate the effect of this detection system. Agarose gel electrophoresis analysis of RT-PCR products found that all neuroblastoma cell lines were positive, normal human peripheral blood was negative, and its sensitivity reached 1 tumor cell/107 peripheral blood polymorphonuclear cells. Clinically, 39 patients with peripheral blood, bone marrow, and histological specimens were confirmed by RT-PCR to detect PGP9.5 as a sensitive and specific method.