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目的研究基质金属蛋白酶2(MMP-2)及内源性基质金属蛋白酶组织抑制剂2(TIMP-2)在慢性心衰中的作用,从分子生物学角度探讨中药不同配伍对慢性心衰心室重构的影响,并进一步阐明其作用机理。方法以冠脉结扎法配合力竭式游泳、减食等方法造成大鼠慢性心衰动物模型,分成模型组、西药组、益气中药组、活血中药组、益气活血中药组、益气活血利水中药组和正常组。利用实时荧光定量PCR技术及SABC免疫组化法检测慢性心衰大鼠MMP-2、TIMP-2表达的变化情况。结果慢性心衰动物模型组大鼠心肌MMP-2表达明显升高,TIMP-2表达降低,与假手术组比较,差异有统计学意义(P<0.01);经过治疗,各用药组大鼠心肌组织MMP-2表达明显降低,TIMP-2表达升高,其中益气活血组、益气活血利水组与西药组比较,差异无统计学意义(P>0.05),且益气活血利水组疗效明显优于益气活血组(P<0.01)。结论益气活血利水中药作用于心肌组织,通过影响MMP-2、TIMP-2的表达,抑制或逆转心室重构的过程,从而达到治疗慢性心衰的目的。
Objective To investigate the role of matrix metalloproteinase 2 (MMP-2) and endogenous tissue inhibitor of metalloproteinase 2 (TIMP-2) in chronic heart failure. To explore the effect of different compatibility of traditional Chinese medicine on ventricular weight Structure, and further clarify its mechanism of action. Methods The animal models of chronic heart failure were induced by coronary ligations combined with exhaustive swimming and diet reduction. The rats were divided into three groups: model group, western medicine group, Yiqi herbs group, Huoxue herbs group, Yiqi Huoxue herbs group, Yiqi Huoxue group Lee Shui medicine group and normal group. The changes of MMP-2 and TIMP-2 expression in chronic heart failure rats were detected by real-time fluorescence quantitative PCR and SABC immunohistochemistry. Results The expression of MMP-2 and the expression of TIMP-2 in myocardium of chronic heart failure model group were significantly higher than those in sham operation group (P <0.01). After treatment, The expression of MMP-2 was significantly decreased and the expression of TIMP-2 was increased. There was no significant difference between Yiqi Huoxue Decoction and Yiqi Huoxue Li Shui Decoction and western medicine group (P> 0.05) Better than the Yiqihuoxue group (P <0.01). Conclusion Yiqi Huoxue Lishui can exert its effect on myocardial tissue, and can inhibit or reverse the process of ventricular remodeling by affecting the expression of MMP-2 and TIMP-2, so as to achieve the goal of treating chronic heart failure.