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为评价Na~+-H~+交换阻滞剂对缺血再灌注损伤的保护作用,在改良的Langendoff装置上采取主动脉逆灌方法,用成年Wistar大鼠的离体鼠心完全停灌60min,然后100%再灌注30min造成再灌注损伤模型。阿米洛利(0.2、20、100μmol/L)和呋喃苯胺酸(10μmol/L)为药物保护组及不加药物的对照组。结果显示:阿米洛利和呋喃苯胺酸对再灌注时心肌收缩力、冠脉流量的恢复及抑制再灌注心律失常的发生有明显的作用,阿米洛利抗心律失常的效果呈明显的剂量依赖性。因此提示具Na~+-H~+交换阻断作用的阿米洛利和呋喃苯胺酸具明显的抗再灌注损伤的作用。
In order to evaluate the protective effect of Na ~ + -H ~ + exchange blocker on ischemia-reperfusion injury, aortic instillation was performed on a modified Langendoff apparatus. The isolated rat heart of adult Wistar rats was stopped for 60min , Followed by 100% reperfusion for 30 minutes to induce a reperfusion injury model. Amiloride (0.2, 20, 100 micromol / L) and furosemide (10 micromol / L) were drug-protective group and non-drug-control group. The results showed that amiloride and furosemide had significant effects on myocardial contractility, coronary flow recovery and inhibition of reperfusion arrhythmia during reperfusion. The anti-arrhythmic effect of amiloride was significantly dose-dependent Sex. Therefore, it is suggested that amiloride and furosemide, which have the blocking effect of Na ~ + -H ~ + exchange, have a significant anti-reperfusion injury effect.