论文部分内容阅读
目的了解深圳市龙岗区涉禽场所外环境禽流感病毒的污染情况,探索影响其病毒核酸检出率的因素。方法 2015年11月—2016年4月,选择龙岗区不同涉禽场所,按月采集不同类型外环境标本,采用RT-PCR方法检测禽流感病毒核酸。结果 590份外环境标本中,215份检出A型流感病毒核酸,A型、H5亚型(均为H5N6)、H9亚型和H7N9禽流感病毒核酸检出率分别为36.44%、18.81%、10.17%和2.37%。非条件多因素Logistic回归分析显示,市场禁售活禽/采取休市措施、市场改造升级、销售冰鲜禽类及其制品可降低外环境标本A型、H5亚型、H9亚型流感病毒核酸的检出率,而小型活禽零售市场、市场通风不良、2016年1月—4月间外环境采样是促进A型、H5亚型和H9亚型流感病毒核酸检出的危险因素。结论深圳市龙岗区H5、H9、H7N9等禽流感病毒的污染广泛,涉禽市场暴露存在人感染禽流感疫情的风险。加强涉禽市场外环境监测力度,全面禁售活禽、推广“冰鲜上市”是降低人禽流感发生风险的关键。
Objective To understand the pollution situation of avian influenza virus outside the environment in Wugong District, Longgang District, Shenzhen, and explore the factors influencing the detection rate of its nucleic acid. Methods From November 2015 to April 2016, different Wading places in Longgang district were selected and different types of environmental samples were collected on a monthly basis. The nucleic acid of the bird flu virus was detected by RT-PCR. Results Among the 590 samples, 215 were positive for nucleic acid detection of influenza A virus, type A and type H5 (all H5N6), H4 subtype and H7N9 were 36.44% and 18.81% respectively, 10.17% and 2.37%. Logistic regression analysis of unconditional multivariate logistic regression showed that the sale of live poultry in the market / the adoption of the market-closed measure, the market transformation and upgrading, and the sale of chilled poultry and their products could reduce the detection of influenza A, H5 and H9 subtype influenza virus nucleic acids While the small poultry retail market and poor ventilation in the market are the risk factors for the detection of influenza A, H5 and H9 subtype influenza viruses in the external environment sampled from January to April in 2016. Conclusion The H5, H9, H7N9 and other avian influenza viruses in Longgang District of Shenzhen City are widely polluted and there is a risk of people being infected with the bird flu. We will step up efforts to monitor the environment outside the wading market, stop the sale of live poultry and promote the listing of chilled fish. This is the key to reducing the risk of human bird flu.