OsEBP-89基因是水稻中一个转录因子编码基因.该基因含有两个内含子.采用RT-PCR和Southern杂交等方法的检测结果表明,在OsEBP-89基因的部分转录本中,115bp长的第1内含子未被剪接.从水稻cDNA文库也筛选到了仍然保留有第1内含子的OsEBP-89基因的cDNA克隆.OsEBP-89基因第1内含子不完全剪接的转录本与正常成熟转录本的比例在水稻不同组织中是不同的.这是真核基因中内含子滞留的一个新的例子. The OsEBP-89 gene is a transcription factor encoding gene in rice, which contains two introns.The results of RT-PCR and Southern blot showed that in the partial transcript of OsEBP-89 gene, 115bp The first intron was not spliced.The cDNA clones of OsEBP-89 gene, which still retained the first intron, were also screened from rice cDNA library.The transcripts with incomplete splicing of the first intron of OsEBP-89 gene and the normal The proportion of mature transcripts is different in different tissues of rice, which is a new example of intron retention in eukaryotic genes.