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目的探讨依达拉奉预处理对小鼠脑缺血再灌注(IR)损伤后皮质一氧化氮合酶(NOS)表达的影响。方法 48只健康ICR小鼠被分为假手术组、对照组和依达拉奉组。依达拉奉组和对照组分别给予依达拉奉3 mg/(kg.d)和同等体积的生理盐水腹腔注射共7 d,然后建立小鼠IR模型;缺血1 h、再灌注24 h时应用2,3,5-氯化三苯基四氮唑(TTC)染色法测量各组脑梗死体积,应用免疫组化法检测各组小鼠皮质神经元型、、诱导型和内皮型NOS(nNOS、iNOS、eNOS)阳性细胞数。结果与假手术组比较,对照组小鼠皮质nNOS、iNOS和eNOS阳性细胞数明显增多(均P<0.05);与对照组比较,依达拉奉组脑梗死体积明显缩小,皮质nNOS和iNOS阳性细胞数明显减少,eNOS阳性细胞数明显增多(均P<0.05)。结论依达拉奉预处理可以影响IR小鼠皮质nNOS、iNOS和eNOS的表达,发挥神经保护作用。
Objective To investigate the effect of edaravone preconditioning on cortical nitric oxide synthase (NOS) expression in mice with cerebral ischemia-reperfusion (IR) injury. Methods Forty-eight healthy ICR mice were divided into sham operation group, control group and edaravone group. Edaravone group and control group were given edaravone 3 mg / (kg.d) and the same volume of saline intraperitoneal injection for 7 days, and then establish the mouse IR model; ischemia 1 h, 24 h reperfusion The volume of cerebral infarction in each group was measured by TTC staining method. Immunohistochemistry was used to detect the neuronal, inducible and endothelial NOS (nNOS, iNOS, eNOS) positive cells. Results Compared with the sham operation group, the number of cortical nNOS, iNOS and eNOS positive cells in the control group was significantly increased (all P <0.05). Compared with the control group, the volume of cerebral infarction in the edaravone group was significantly reduced, and the cortical nNOS and iNOS positive The number of cells was significantly decreased and the number of eNOS positive cells was significantly increased (all P <0.05). Conclusion Edaravone pretreatment can affect the expression of nNOS, iNOS and eNOS in the cortex of IR mice and exert the neuroprotective effect.