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建立了荧光假单胞菌发酵液中谷胱甘肽的高效液相色谱测定方法。采用Symmetry C18柱分离,流动相为磷酸二氢钠(p H=3)和0.1%的辛烷磺酸钠混合溶液:甲醇=92.5:7.5(v/v),流速0.8 m L/min,柱温30℃,紫外检测器,检测波长210 nm。结果显示,谷胱甘肽在10~160 mg/L浓度范围内与峰面积有良好的线性关系(线性系数为0.9999),精密度、稳定性、重复性以及加标回收率实验的RSD值均符合检测要求。方法能快捷准确检测出荧光假单胞菌发酵液中还原型谷胱甘肽的含量。
A method for the determination of glutathione in the fermentation broth of Pseudomonas fluorescens was established. The mixture was separated on a Symmetry C18 column using a mixture of sodium dihydrogen phosphate (p H = 3) and 0.1% sodium octane sulfonate in methanol: 92.5: 7.5 (v / v) at a flow rate of 0.8 mL / Temperature 30 ℃, UV detector, detection wavelength 210 nm. The results showed that glutathione had a good linear relationship with the peak area (linearity 0.9999) in the concentration range of 10 ~ 160 mg / L, and the RSD values of precision, stability, repeatability and spike recoveries Meet the testing requirements. The method can quickly and accurately detect the content of reduced glutathione in the fermentation broth of Pseudomonas fluorescens.