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目的探讨端粒酶在胃癌及癌前组织中的活性表达。方法采用端粒酶反复扩增法（ＴＲＡＰ）检测了１７６例不同病变胃粘膜组织端粒酶活性，其中慢性萎缩性胃炎（ＣＡＧ）５７例、肠上皮化生（ＩＭ）１８例、异型增生（Ｄｙｓ）８例及胃癌（ＧＣ）６５例（包括３例早期胃癌）。结果ＣＡＧ、ＩＭ、Ｄｙｓ及ＧＣ端粒酶阳性检出率分别为２４．６％、３８．５％、３７．５％及９２．３％，而正常组织（ＮＴ）未检出端粒酶活性，明显低于以上各组（Ｐ＜０．０１～０．０５）。癌组织端粒酶阳性率亦明显高于ＣＡＧ、ＩＭ及Ｄｙｓ组（Ｐ＜０．０１）；端粒酶阳性检出率与患者性别、肿瘤大小、浸润深度、大体类型、分化程度、有无淋巴结转移及临床分期无明显相关性。结论端粒酶不仅在胃癌组织中可以检测得到，而且在胃粘膜癌前病变或疾病中亦有表达。
Objective To investigate the expression of telomerase activity in gastric cancer and precancerous tissues. Methods The telomerase activity of gastric mucosa was detected in 176 patients with different pathological changes using telomerase repeat amplification assay (TRAP). Among them, there were 57 cases of chronic atrophic gastritis (CAG), 18 cases of intestinal metaplasia (IM), and dysplasia ( Dys) 8 cases and gastric cancer (GC) 65 cases (including 3 cases of early gastric cancer). Results The positive detection rates of CAG, IM, Dys, and GC telomerase were 24.6%, 38.5%, 37.5%, and 92.3%, respectively, while no telomerase activity was detected in normal tissues (NT). , significantly lower than the above groups (P <0.01 to 0.05). The positive rate of telomerase in cancer tissue was also significantly higher than that in CAG, IM, and Dys groups (P<0.01). The positive rate of telomerase detection was related to the patient’s gender, tumor size, depth of invasion, general type, degree of differentiation, presence or absence of telomerase. There was no significant correlation between lymph node metastasis and clinical stage. Conclusion Telomerase can be detected not only in gastric cancer but also in gastric precancerous lesions or diseases.