目的:观察地塞米松(dexamethasone,Dex)对胰岛β细胞凋亡的影响及NADPH氧化酶(Nox)表达水平的变化。方法:将大鼠胰岛β细胞系INS-1细胞分为正常对照组及不同浓度的地塞米松处理组。培养48 h后MTT法检测细胞增殖情况;Annexin V-FITC/PI双染流式细胞术检测细胞凋亡情况;real-time PCR检测Nox1、Nox3、Nox4和p47phoxmRNA表达水平;荧光法检 Objective: To observe the effect of dexamethasone (Dex) on the apoptosis of pancreatic β cells and the change of NADPH oxidase (Nox) expression. Methods: Rat pancreatic β-cell line INS-1 cells were divided into normal control group and different concentrations of dexamethasone treatment group. Cell proliferation was measured by MTT assay after 48 h of culture; apoptosis was detected by flow cytometry with Annexin V-FITC / PI; the expression of Nox1, Nox3, Nox4 and p47phox mRNA was detected by real-time PCR;