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目的探讨SATB1异常表达与结肠癌发生、发展及预后的关系。方法分别用RT-PCR和Western blot法检测SATB1在25例结肠癌活检组织及相应癌旁正常组织中mRNA和蛋白表达水平;采用免疫组化检测58例结肠癌石蜡组织切片中SATB1的表达,分析其与结肠癌临床病理因素的关系及预后意义。结果 SATB1 mRNA和蛋白在92%(23/25)结肠癌活检组织中的表达水平高于其癌旁正常组织;58例结肠癌石蜡组织中,SATB1蛋白强阳性率为46.6%(27/58)。统计学分析结果显示,SATB1强阳性与结肠癌Dukes分期和远处转移密切相关,而与性别、年龄、病理分级、肿瘤大小、淋巴结转移无显著相关性。结论 SATB1在结肠癌中高表达,其表达水平与肿瘤进展相关,可作为预测结肠癌进展和转移的分子标记物。
Objective To investigate the relationship between the abnormal expression of SATB1 and the occurrence, development and prognosis of colon cancer. Methods The mRNA and protein expressions of SATB1 in 25 cases of colon cancer biopsies and adjacent normal tissues were detected by RT-PCR and Western blot respectively. The expression of SATB1 was detected by immunohistochemistry in 58 cases of colon cancer paraffin sections. Its relationship with clinicopathological factors and prognostic significance of colon cancer. Results The SATB1 mRNA and protein expression in 92% (23/25) colon cancer biopsy tissues were higher than those in normal tissues. The strong positive rate of SATB1 protein in 58 colon cancer paraffin tissues was 46.6% (27/58) . Statistical analysis showed that strong positive SATB1 was closely related to staging and distant metastasis of Dukes, but not to gender, age, pathological grade, tumor size and lymph node metastasis. Conclusions SATB1 is highly expressed in colon cancer and its expression level correlates with tumor progression. It may serve as a molecular marker for predicting colon cancer progression and metastasis.