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目的:筛选分离纯化骨碎补中柚皮苷的最佳树脂型号及最佳工艺。方法:通过静态、动态吸附和解吸相结合的方法,以柚皮苷的吸附率、解吸率为评价指标综合评判确定树脂型号;通过考察上柱药材量、上样流速考察、洗脱乙醇浓度、洗脱溶剂用量,最终确定了纯化工艺条件。结果:HPD-100型大孔吸附树脂分离纯化骨碎补中柚皮苷效果最好,其最佳工艺条件为:以药材∶大孔吸附树脂为1∶1(质量比)上样,上样流速为3 BV.h-1,以8倍柱体积水洗,继以14倍柱体积50%乙醇洗脱。结论:采用HPD-100型大孔吸附树脂分离纯化骨碎补中柚皮苷效果良好。
OBJECTIVE: To screen the best resin type and the best technology for isolation and purification of naringin in Gusui. Methods: Through the combination of static, dynamic adsorption and desorption methods, the resin model was determined by the evaluation rate of naringin adsorption rate and desorption rate; the amount of drug in the column, the sample flow rate, the concentration of elution ethanol were examined. The amount of elution solvent used to finalize the purification process conditions. RESULTS: HPD-100 macroporous adsorption resin had the best effect on separation and purification of naringin from Rhizoma Drynariae. The optimum conditions were: loading 1:1 mass ratio of medicinal material: macroporous adsorption resin and loading sample. The flow rate was 3 BV.h-1, washed with 8 column volumes of water, followed by 14 column volumes of 50% ethanol. Conclusion: It is effective to separate and purify naringin from Rhizoma Drynaria with HPD-100 macroporous adsorption resin.