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DPPA2(Developmental Pluripotency-Associated gene2)是近年来发现的在多能性细胞中特异表达的一个基因,它被认为参与维持干细胞的“干性”.但目前为止,并没有关于该基因在人类胚胎干细胞(human embryonic stem cells,hESCs)分化过程中的表达情况的报道,其功能也尚不清楚.通过Real-time PCR对DPPA2基因在hESCs分化过程中的表达情况进行分析,此外还对其在异常核型hESCs,人类胚胎癌细胞(human embryonic carcinoma cells,hECCs)NTERA-2以及其它5种癌细胞中的表达情况进行检测.结果表明DPPA2基因在hESCs中特异表达,其表达水平随着hESCs的分化而显著下调.该基因在异常核型hESCs和NTERA-2细胞中也有表达,但在其它肿瘤细胞中未检测到该基因的表达.此外,以EGFP-N1系统为基础的亚细胞信号定位结果表明,DPPA2是一个核蛋白.这些结果提示,DPPA2基因可能在维持hESCs特性的过程中发挥着重要的作用.
DPPA2 (Developmental Pluripotency-Associated gene2) is a gene that is specifically expressed in pluripotent cells in recent years and is considered to be involved in the maintenance of stem cells. "So far, however, The function of DPPA2 gene in the process of hESCs differentiation was analyzed by Real-time PCR.It was also reported that the expression of DPPA2 gene in hESCs during the process of differentiation of human embryonic stem cells (hESCs) The results showed that DPPA2 gene was expressed specifically in hESCs, and the expression level of DPPA2 was increased with the expression of hESCs in hESCs, hTERCs, NTERA-2 cells and other five kinds of cancer cells. Differentiated and downregulated.The gene was also expressed in abnormal karyotype hESCs and NTERA-2 cells, but not in other tumor cells.In addition, EGFP-N1 system-based subcellular signal localization Indicating that DPPA2 is a nuclear protein.These results suggest that DPPA2 may play an important role in maintaining the properties of hESCs.