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一、孵化酶的分泌鱼类孵化的最终过程是卵膜溶解。溶解卵膜的孵化酶是由单细胞的孵化腺分泌的。古田发现鳉的孵化腺位于咽头壁,在孵化腺溶解之前,胚体开始呼吸运动(鳃盖运动)这时,如果把胚体放入M/4 KCL溶液中,就抑制了鳃盖运动,孵化腺不再分泌。但是把胚体放入0.1％—0.2％的佛罗那——苏打液中,尽管胚体受到麻醉,却因鳃盖运动没有被抑制仍就能孵化。即使把玻璃毛细管插入口腔内,喷入水流,也发生孵化腺的溶解。上述事实说明:孵化腺的分泌是由于鳃盖运动引起水流的机械刺激的结果。
First, the secretion of hatching enzyme The final process of fish hatching is the dissolution of the egg membrane. Egg-dissolving hatch enzymes are secreted by single-cell hatching glands. Gutian found hatched gland’s hatching gland is located in the pharyngeal wall, before the hatch gland dissolves, the embryo body began to breathe (gill cover movement) At this time, if the embryo body into M / 4 KCL solution, inhibit the gill cover movement, Hatching glands no longer secreted. However, placing the embryoid body in 0.1% -0.2% Florida-soda resulted in hatching even though the embryo was anesthetized but the gill movement was not inhibited. Even if the glass capillary is inserted into the oral cavity and injected into the water stream, hatching glands are dissolved. The above fact indicates that the secretion of the hatching gland is the result of the mechanical stimulation of the water flow caused by the movement of the gill cover.