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目的 :观察小鼠胚胎干细胞在裸鼠眼内生长的形态变化。方法 :将胚胎干细胞在体外培养 ,并维持在未分化的状态 ,再移植到Balb/c裸小鼠眼内。 6 - 45d处死裸鼠 ,形态学和免疫组化观察其变化。结果 :胚胎干细胞移植到裸小鼠右眼后 ,在眼前房和玻璃体腔内生长。形态学检查在前房和玻璃体腔内见到各种形态的细胞 ,出现囊样、腺样、管样、菊花样等结构。免疫组化NSE染色多数细胞呈强阳性反应 ,部分细胞GFAP也呈中强度阳性反应。结论 :胚胎干细胞D3株在Balb/c裸小鼠眼内发育分化成了髓上皮瘤样组织
Objective: To observe the morphological changes of mouse embryonic stem cells in the eye of nude mice. Methods: Embryonic stem cells were cultured in vitro and maintained in an undifferentiated state and then transplanted into the eye of Balb / c nude mice. The nude mice were sacrificed at 6 - 45 days, and their morphological and immunohistochemical changes were observed. Results: Embryonic stem cells were transplanted into the right eye of nude mice and grew in the anterior chamber and vitreous cavity. Morphological examination in the anterior chamber and vitreous cavity to see a variety of forms of cells, there cystoid, adenoid, tubular, chrysanthemum and other structures. Most of the cells in NSE stained immunohistochemistry showed a strong positive reaction, some of the cells also showed a moderate positive GFAP. CONCLUSION: Embryonic stem cell D3 strain differentiated into myeloid tumor-like tissue in Balb / c nude mice