【摘 要】
：
目的:建立灵敏、特异的人血浆AVP放射免疫测定法.方法:用氨胺-T法标记AVP,BSA终止反应,经SephadexG-25柱纯化,获高比放射性125I-AVP,血浆标本经C18 Sep-Pak柱纯化后进行放免
【机 构】
：
上海第二医科大学上海瑞金医院上海市内分泌研究所 200025
论文部分内容阅读
目的:建立灵敏、特异的人血浆AVP放射免疫测定法.方法:用氨胺-T法标记AVP,BSA终止反应,经SephadexG-25柱纯化,获高比放射性125I-AVP,血浆标本经C18 Sep-Pak柱纯化后进行放免分析.结果:该方法灵敏度为0.7pg,回收率93.5±8.1%;批间变异系数13%,当血浆AVP浓度为3.0pg/ml和11.0pg/ml时,批内变异系数分别为11%和6.4%.临床应用结果证实:隔夜禁饮,正常人清晨空腹的血浆渗透压为290.0±7.3mosm/kgH2O时,血浆AVP浓度为3.8±1.9pg/ml;本法能准确诊断不适当的抗利尿激素分泌和尿崩症,将典型的中枢性尿崩症与其他低渗性多尿症区分开来.结论:本方法适用于临床及科研.
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