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趋化因子是—种细胞因子 ,它是由组织细胞和炎性细胞衍生的、具有白细胞趋化活性的肽分子。论文确定了筛选趋化因子的实验方法趋化小室实验及其最佳实验条件 :以含 0 .1% BSA(牛血清蛋白 )的 RPMI16 4 0培养基为阴性溶剂 ,以趋化因子 Fmlp为阳性对照 ,白细胞浓度为 1.6×10 3/ μL 等。在上述条件下 ,对从 10种蛇毒中分离的 32 7个样品进行筛选 ,得到有趋化活性的样品 5个 ,经体外细胞毒性实验验证 ,其中 4个对细胞无毒性。趋化因子在抗肿瘤和AIDS病的防治等方面具有药用价值。
Chemokines are cytokines, which are peptide molecules derived from tissue cells and inflammatory cells and having leukocyte chemotactic activity. The paper identified the chemotactic factor screening experiment method chemotaxis chamber experiments and the best experimental conditions: with 0.1% BSA (bovine serum albumin) RPMI1640 medium as a negative solvent to chemokine Fmlp positive In contrast, leukocyte concentration was 1.6 × 10 3 / μL and the like. Under the above conditions, 32 7 samples isolated from 10 species of snake venom were screened, and 5 samples with chemotactic activity were obtained. Four of them were non-toxic to cells by in vitro cytotoxicity test. Chemokines have medicinal value in the prevention and treatment of anti-tumor and AIDS diseases.