论文部分内容阅读
本文对大骨节病区和非病区产的玉米用电子捕获检测器气相色谱仪测定丁烯酸内酯(Bu)的方法进行了测定。病区玉米提取液向气相色谱仪(GC)进样后,常可出现一个与 Bu 标准毒素的保留时间相同的色谱峰。在40份病区玉米样品中检出了这种疑为 Bu 的物质有27份(67.5%)。非病区玉米样品32份中检出的只有3份(9.4%),两者差别十分显著(P=0.004×10~(-4))。这一疑为 Bu 的物质及其紧邻的色谱峰经过气相色谱-质谱联用的分析鉴定,它们的分子量(143及132+X)与 Bu(141)接近,而它们的碎片离子的质荷比(m/z)及离子峰的相对强度与 Bu 的有所不同,因此玉米提取液中出现的与 Bu 保留时间相同的色谱峰乃是一种与 Bu 不同的物质。至于它们的化学结构、来源以及是否与大骨节病的发病有何种联系,尚待进一步探究。
In this paper, the method of determination of butenolide (Bu) by GC with electron capture detector was developed for the detection of maize in the bardiosis and non-diseased areas. Ward corn extract into the gas chromatograph (GC) injection, often with a standard Bu toxin retention time of the same peak. 27 (67.5%) of these suspected Bu were detected in 40 ward corn samples. There were only 3 (9.4%) out of 32 samples of non-ward corn sample, the difference was significant (P = 0.004 × 10 -4). The suspected Bu and its adjacent peaks were identified by gas chromatography-mass spectrometry. Their molecular weights (143 and 132 + X) were close to those of Bu (141), and the mass-to-charge ratio of their fragment ions (m / z) and ion peak relative intensity and Bu different, so the emergence of the corn extract and Bu retention time of the same peak but a Bu and a different substance. As for their chemical structure, source and whether the incidence of Kashin-Beck disease has any connection, yet to be further explored.