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Recombinant plasmid pcDNA-L1 was constructed by inserting HPV16-L1 gene fragment into eukaryotic expression plasmid pcDNA3.1. pcDNA-L1 was transfected into mammalian cells Cos-7 and the expression of HPV16-L1 protein was testified by immunohistochemistry(IHC). Then the recombinant plasmid was directly injected into the quadriceps muscle of BALB/c mice. Antibodies against HPV16-L1 in the immunized mice were positively detected by immunodot and IHC at 28 and 41 days after the last immunization.
Recombinant plasmid pcDNA-L1 was constructed by inserting HPV16-L1 gene fragment into eukaryotic expression plasmid pcDNA3.1. PcDNA-L1 was transfected into mammalian cells Cos-7 and the expression of HPV16-L1 protein was testified by immunohistochemistry (IHC). Then the recombinant plasmid was directly injected into the quadriceps muscle of BALB / c mice. Antibodies against HPV16-L1 in the immunized mice were positively detected by immunodot and IHC at 28 and 41 days after the last immunization.