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以大花蕙兰与朱砂兰杂交种子萌发后形成的根状茎为试验材料,采用KT、6-BA和NAA三种外源激素对杂交兰根状茎进行实验,比较不同激素对根状茎增殖和分化的影响。单因素实验中,随着激素KT、6-BA浓度的增加,杂交兰根状茎的增殖率和分化率逐渐增加,但不成正比例关系。NAA浓度一定时,6-BA浓度为3 mg/L时,杂交兰根状茎的平均增殖率最高达到290%,6-BA浓度为4 mg/L时,根状茎的平均分化率最高达到85.64%;KT浓度为2.5 mg/L时,平均增殖率最高为300%;KT浓度为3.0 mg/L时,根状茎的平均分化率最高为87.89%。三种激素复合添加对杂交兰根状茎增殖和分化效果最佳。增殖效果最佳的培养基为1/2 MS+6-BA 3.0 mg/L+KT 2.0 mg/L+NAA 0.2 mg/L+琼脂粉7.0 g/L+香蕉80 g/L+蔗糖30 g/L+活性炭1 g/L,根状茎粗壮且长,增殖率高。分化效果最佳培养基为1/2 MS+6-BA 4.0 mg/L+KT 2.5 mg/L+NAA 0.2 mg/L+琼脂粉7.0 g/L+香蕉80 g/L+蔗糖30 g/L+活性炭1 g/L,分化苗生长良好,叶色深绿。本研究为杂交优株实现工厂化育苗奠定了一定的理论基础。
Rhizomes formed after hybrid seeds germination of Cymbidium hybridum and Zhujalan were used as experimental materials. Three rhizomes of KT, 6-BA and NAA were used to test rhizomes. The effects of different hormones on rhizome Effects of proliferation and differentiation. In single factor experiments, the proliferation rate and differentiation rate of rhizomes of hybrid rhizome increased with the increase of KT and 6-BA concentrations, but not in a proportional relationship. When the concentration of NAA was 6 mg / L, the average multiplication rate of rhizome was up to 290% when the concentration of 6-BA was 3 mg / L and that of rhizome reached the highest when the concentration of 6-BA was 4 mg / L 85.64%. When the concentration of KT was 2.5 mg / L, the average proliferation rate was up to 300%. When KT was 3.0 mg / L, the average rate of rhizome differentiation was 87.89%. The three hormones were the best for hybrid rhizome proliferation and differentiation. The best medium for proliferation was 1/2 MS + 6-BA 3.0 mg / L + KT 2.0 mg / L + NAA 0.2 mg / L + agar 7.0 g / L + banana 80 g / L + sucrose 30 g / L + g / L, rhizome thick and long, high proliferation rate. The optimum medium for differentiation was 1/2 MS + 6-BA 4.0 mg / L + KT 2.5 mg / L + NAA 0.2 mg / L + agar 7.0 g / L + banana 80 g / L + sucrose 30 g / L + / L, differentiation of seedlings grow well, dark green leaves. This study laid a certain theoretical foundation for hybrid plant superior seedling.