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目的利用筛选的党参多态性微卫星引物进行党参群体遗传多样性研究。方法利用磁珠富集法分离党参核基因组微卫星引物,并选取党参4个野生居群48个样品进行遗传多样性分析。结果筛选出了10个能成功扩增党参样品的微卫星引物;这些引物扩增产物的等位基因数目为5～14个,观察杂合度范围为0.446～0.833,期望杂合度范围为0.697～0.895;使用4个党参近缘物种(轮叶党参、球花党参、鸡蛋参和长叶党参)进行引物通用性检测,发现有5对引物(Cpi01、Cpi04、Cpi06、Cpi07和Cpi08)能同时在4个物种中成功扩增。结论筛选出的多态性微卫星引物能用于党参的遗传多样性和群体遗传结构研究,同时也能够用于党参属其他近缘物种的群体遗传学研究。
Objective To study the genetic diversity of Codonopsis pilosula using screened CAG polymorphic microsatellite primers. Methods The microsatellite DNA primers of Corynebacterium pyogenes were isolated by magnetic beads enrichment and 48 samples of 4 wild populations from Codonopsis pilosula were selected for genetic diversity analysis. Results Ten microsatellite primers were successfully screened. The number of alleles amplified by these primers ranged from 5 to 14, the observed heterozygosity ranged from 0.446 to 0.833, and the expected heterozygosity ranged from 0.697 to 0.895 Four primers (Cpi01, Cpi04, Cpi06, Cpi07 and Cpi08) were used to test the commonality of the primers in four Codonopsis species (Codonopsis glauca, Codonopsis pilosula, Echinacea angustifolia and Codonopsis pilosula) A species of successful expansion. Conclusion Polymorphic microsatellite primers selected can be used for genetic diversity and population genetic structure of Codonopsis pilosula and Codonopsis pilosula as well as for population genetics of other related species.