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探讨趋化因子生长调节致癌基因α(GROα)在低密度脂蛋白诱导动脉粥样硬化过程中的作用。采用人脐带静脉内皮细胞和单核细胞系细胞———U 937细胞。结果显示 ,内皮细胞暴露于氧化低密度脂蛋白后显著上调GROαmRNA ;氧化低密度脂蛋白时间、浓度依赖性地上调内皮细胞表面GROα的蛋白表达 ,但溶液中的GROα蛋白并没有显著改变。内皮细胞表面GROα蛋白上调增加了单核细胞粘附到内皮细胞 ,且GROα抗体抑制此粘附过程。提示氧化低密度脂蛋白功能性上调人脐带静脉内皮细胞GROα的表达。
To investigate the role of chemokine growth-regulated oncogene α (GROα) in LDL-induced atherosclerosis. Human umbilical vein endothelial cells and monocyte-cell U-937 cells were used. The results showed that endothelial cells exposed to oxidized low-density lipoprotein significantly up-regulated GROα mRNA; oxidized low-density lipoprotein increased the expression of GROα on the surface of endothelial cells in a time- and concentration-dependent manner, but the GROα protein in solution did not change significantly. The up-regulation of GROα protein on the surface of endothelial cells increases the adhesion of monocytes to endothelial cells, and GROα antibodies inhibit this process of adhesion. It was suggested that oxidized low-density lipoprotein functionally up-regulates GROα expression in human umbilical vein endothelial cells.