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目的了解北京地区住院急性呼吸道感染(ARI)患儿的病毒病原情况。方法取1260例年龄14岁以下住院ARI患儿的鼻咽深部分泌物,用间接免疫荧光及病毒分离法检测呼吸道合胞病毒(RSV)、流感病毒A、B型、副流感病毒1、2、3型及腺病毒等7种常见呼吸道病毒。用逆转录聚合酶链反应(RT-PCR)法对其中490例患儿标本进行肠道病毒(EV)检测。结果1.ARI1260例中,43.33%检测到了病毒病原,7种常见呼吸道病毒检出率36.19%,RSV阳性率最高(23.97%),以冬春季为著,88.08%的RSV阳性为3岁以下小儿。2.EV阳性率16.33%。3.19例存在2种病毒混合感染,均出现在冬春季,16例为RSV并EV感染。4.入选的510例急性呼气性喘息患儿中,3岁以下RSV阳性率最高(43.20%),3岁以上EV阳性率最高(36.11%)。结论1.RSV是北京地区冬春季婴幼儿ARI的主要病原。2.冬春季EV可并RSV等其他呼吸道病毒感染。3.RSV及EV是引起小儿急性喘息性疾病的主要病毒病原。
Objective To understand the viral pathogens in children with acute respiratory infection (ARI) in Beijing. Methods Deep venous nasopharyngeal secretions from 1260 ARI children under 14 years of age were collected. Respiratory syncytial virus (RSV), influenza A, B, parainfluenza virus 1, 2 and 2 were detected by indirect immunofluorescence and virus isolation. 3 types and adenovirus 7 kinds of common respiratory viruses. 490 of them were tested for enterovirus (EV) by reverse transcription-polymerase chain reaction (RT-PCR). Among the 1260 cases of ARI, 43.33% detected the virus pathogens, the detection rate of 7 kinds of common respiratory viruses was 36.19%, the highest positive rate of RSV (23.97%) was in winter and spring, 88.08% of RSV positive were children under 3 years old . 2.EV positive rate of 16.33%. There were two kinds of virus mixed infection in 3.19 cases, both in winter and spring, 16 cases were RSV and EV infection. Among the 510 patients with acute asthmatic wheeze, the positive rate of RSV was the highest (43.20%) under 3 years of age and the highest was EV (3) (36.11%). RSV is the main pathogen of ARI in winter and spring in Beijing. 2. Winter and spring EV and RSV and other respiratory virus infection. RSV and EV are the main viral pathogens in children with acute wheezing diseases.