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氨基酸的手性拆分在生命的起源、发育、病变及衰老研究中均有重要的意义．虽然已有基于色谱和毛细管电泳的拆分方法，但由于氨基酸种类繁多，性质各异，因而具有广泛拆分能力的普适性拆分方法还很有限．组合运用手性选择剂是提高手性分离选择性和分离度的一种有效的方法［1-3]．为此，我们以毛细管电泳－激光诱导荧光检测（CE－LIF）为分析手段，研究了手性选择剂的添加组合，获得了一些具有一定广谱拆分性能的二元手性选择剂协同体系．本文以β-环糊精（β-CD）和牛磺胆酸钠（STC）为拆分体系，对20种氨基酸的异硫氰酸酯荧光素（FITC）衍生物进行手性拆分，分离度均在1．9
Chiral resolution of amino acids in the origin of life, development, disease and aging are of great significance. Although chromatographic and capillary electrophoresis-based resolution methods exist, universal resolution methods with a wide range of resolution capabilities are limited due to the wide variety and nature of amino acids. The combination of chiral selectors is an effective method to improve the selectivity and resolution of chiral separation [1-3]. To this end, we used capillary electrophoresis - laser-induced fluorescence detection (CE-LIF) as the analytical means, we studied the addition of chiral selectors combination obtained a number of some broad-spectrum binary chiral selector cooperative system . In this paper, β-cyclodextrin (β-CD) and sodium taurocholate (STC) as the resolution system, the 20 amino acids of isothiocyanate fluorescein (FITC) derivatives were chiral resolution, resolution All at 1.9