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研究补光时间与光质对设施作物抗衰老及抗氧化能力的影响,对于充实光质效应理论和优化补光技术有重要意义。研究了补光2、4、8h及蓝光(B)、红光(R)、白光(W)和红蓝复合光(R∶B=7∶3和8∶2)对温室黄瓜幼苗光合色素、丙二醛(MDA)、可溶性蛋白含量及抗氧化能力的影响。结果表明:与自然光照相比,补光可显著提高黄瓜幼苗叶片叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量,其中以补光4h红蓝复合光处理(7R/3B和8R/2B)效果最为显著;补光可提高黄瓜幼苗可溶性蛋白含量,其中,以蓝光效果最好。补照4h蓝光可以明显提高黄瓜幼苗叶片可溶性蛋白的含量及保护酶(过氧化物酶POD、超氧化物歧化酶SOD、抗坏血酸过氧化物酶APX、过氧化氢酶CAT)活性,并且使MDA的含量保持相对较低的水平,从而可以有效延缓叶片衰老。
Studying the effects of fill light time and light quality on the anti-aging and anti-oxidative capacity of facility crops is of great significance to enrich the theory of light quality effect and optimize the fill light technology. The effects of supplemental light 2,4,8h and blue light (B), red light (R), white light (W) and red and blue light (R: B = 7: 3 and 8: 2) on photosynthetic pigments, Malondialdehyde (MDA), soluble protein content and antioxidant capacity. The results showed that supplemental light could significantly increase the content of chlorophyll a, chlorophyll b, total chlorophyll and carotenoid in cucumber seedling leaves compared with the natural light, and the effect of 7R / 3B and 8R / 2B treatments The most significant; fill light can improve the soluble protein content of cucumber seedlings, of which the best blue light. Supplemental 4h blue light could significantly increase the content of soluble protein and activities of protective enzymes (peroxidase POD, superoxide dismutase SOD, ascorbate peroxidase APX, catalase CAT) in cucumber seedling leaves, The content remains relatively low level, which can effectively delay leaf senescence.