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近年研究证明,脑内5-羟色胺(5-HT)的消长与针刺镇痛有着密切的关系。脑内5-羟色胺来自5-羟色胺能神经元。经荧光组织化学研究证明,5-羟色胺能神经元主要集中于脑干内的中缝核群。并表明中缝背核(NRD)和中缝中央上核(NCS)是5-羟色胺能上行系统的重要来源。据报道,在针刺镇痛中,电刺激NRD可以提高痛阈并增强针刺镇痛效应,毁损NRD能明显降低针刺镇痛作用。由此可见,NRD为中枢神经系统中重要镇痛结构之一。我们研究NRD传出性联系之后,应用辣根过氧化物酶(HRP)逆行性传递法进一步研究其传入,以观察NRD与脑内各级水平和镇痛有关核团的联系,当可为功能研究及其作用环路提供一定形态学的根据。 用大鼠28只,在乌拉坦腹腔麻醉下,经定向仪导入HRP,选择其中定位较准确的24只进行分析。应用微量注射法和微电泳法导入HRP,其中n只用于垂直入路,13只经小脑斜行入路。动物存活24～48小时后,经灌注固定,冰冻切片,应用Graham及Karnovsky所介绍的二氨基苯胺棕色反应法显色。
In recent years, studies have shown that brain 5 - HT (5-HT) and acupuncture analgesia has a close relationship. Serotonin in the brain is derived from serotonergic neurons. Fluorescent histochemical studies have shown that serotonergic neurons are mainly concentrated in the brain stem in the middle of the nuclear population. And suggest that the dorsal raphe nucleus (NRD) and the midbrain superior central nucleus (NCS) are important sources of serotonergic upstream systems. It has been reported that in the acupuncture analgesia, electrical stimulation of NRD can increase the pain threshold and enhance the acupuncture analgesic effect, NRD damage can significantly reduce the acupuncture analgesic effect. Thus, NRD is one of the major analgesic structures in the central nervous system. After studying the outbreaks of NRD, we used HRP retrograde transfer to further investigate the association of NRD with various levels of the brain and the associated pain-relieving nucleus, Functional studies and their role Loop provides some basis for morphology. Twenty-eight rats were anesthetized with urethane under anesthesia, HRP was induced by directional instrument, and 24 of them were selected for accurate analysis. HRP was introduced by microinjection and micro-electrophoresis, of which n was used for vertical approach and 13 for oblique approach. Animals survived for 24-48 hours, fixed by perfusion and frozen sections were developed using the diaminoaniline brown reaction described by Graham and Karnovsky.