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肺泡Ⅱ型上皮细胞在肺缺血病理过程中具有重要作用。为研究缺血对陷窝蛋白-1在肺泡Ⅱ型上皮细胞A549脂滴定位的影响,利用已经建立的脂滴纯化方法,纯化得到肺泡Ⅱ型上皮细胞A549的脂滴,并在脂滴上发现了陷窝蛋白-1。在A549细胞缺血模型中发现缺血可以导致陷窝蛋白-1从细胞膜移动到脂滴。人肺组织脂滴纯化实验也证实缺血可以刺激陷窝蛋白-1从细胞膜移动到脂滴。这一发现将为肺缺血机制的研究提供新的思路。
Alveolar type Ⅱ epithelial cells play an important role in the pathological process of pulmonary ischemia. In order to study the effect of ischemia on the lipid droplet localization of lactein-1 in A549 alveolar type II cells, the lipid droplets of A549 alveolar type II epithelial cells were purified by using the established lipid droplet purification method and found on lipid droplets Lacnt-1. Ischemia was found in the A549 cell-ischemia model that resulted in the movement of the caveolin-1 from the cell membrane to the lipid droplets. Lipid purification experiments in human lung tissue also confirmed that ischemia can stimulate the movement of the nestin-1 from the cell membrane to the lipid droplets. This discovery will provide a new way for the research of lung ischemia mechanism.