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目的:研究临床常用抗菌药物亚胺培南、哌拉西林/他唑巴坦对嗜麦芽窄食单胞菌产生L2β-内酰胺酶的诱导作用,指导临床抗菌药物的使用。方法法:应用实时荧光定量PCR方法,研究0、1/4×MIC、1×MIC、4×MIC四种浓度的亚胺培南、哌拉西林/他唑巴坦的诱导作用,观察前后L2基因的mRNA转录水平及变化。结果:1×MIC浓度的哌拉西林/他唑巴坦诱导作用最强,亚胺培南无论任何浓度其诱导作用均较弱。结论:抗菌药物亚胺培南、哌拉西林/他唑巴坦对L2β-内酰胺酶的产生具有明显诱导作用,其中酶抑制剂复合制剂作用最强,临床上应避免长期应用该类药物,以免进一步诱导嗜麦芽窄食单胞菌多药耐药性的产生。
Objective: To study the induction of L2β-lactamase produced by clinically used antibiotics imipenem and piperacillin / tazobactam on Stenotrophomonas maltophilia and to guide the use of clinical antibacterials. Methodology: Induction of imipenem and piperacillin / tazobactam at 0,1 / 4 × MIC, 1 × MIC, 4 × MIC was studied by real-time fluorescence quantitative PCR. Gene mRNA transcription level and changes. Results: Piperacillin / tazobactam at the concentration of 1 × MIC showed the strongest induction and imipenem at any concentration showed weaker induction. Conclusion: The antibacterials imipenem and piperacillin / tazobactam have obvious inducing effects on the production of L2β-lactamase. Among them, the enzyme inhibitor combination has the strongest effect, and should be avoided in the long term. So as to avoid further induction of multi-drug resistance of Stenotrophomonas maltophilia.