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目的观察补骨脂、双氢青蒿素及二者配伍对隐孢子虫的杀灭效果。方法地塞米松磷酸钠注射液腹股沟皮下注射诱导建立隐孢子虫感染模型,随机分成正常组A、阳性对照组B、补骨脂治疗组C、双氢青蒿素治疗组D以及二者配伍治疗组E,并按治疗1～2w时间分成A1、A2;B1、B2;C1、C2;D1、D2;E1、E2共计10组。观察各组小鼠肠壁黏膜组织病理变化及超微结构改变。结果经过1、2w的治疗,所有药物治疗组小鼠的粪便卵囊量与阳性对照组相比,均明显减少(P<0.05),小肠黏膜组织病理及超微结构显示小肠上皮处于修复中,并且合剂治疗组疗效明显优于单剂治疗组(P<0.05)。结论中药补骨脂和双氢青蒿素对隐孢子虫均有抑杀作用,但以后者杀虫效果明显。二者协同作用,共同参与宿主的免疫调节和炎症修复过程。
Objective To observe the killing effect of psoralen, dihydroartemisinin and their compatibility on Cryptosporidium parvum. Methods The model of Cryptosporidium infection induced by subcutaneous inguinal injection of dexamethasone sodium phosphate injection was established and randomly divided into normal group A, positive control group B, psoralen group C, dihydroartemisinin group D and the combination of the two Group E, according to the treatment of 1 ~ 2w time into A1, A2; B1, B2; C1, C2; D1, D2; E1, E2 total of 10 groups. Pathological changes and ultrastructural changes of intestinal mucosa in mice were observed. Results After 1 and 2 weeks of treatment, the amount of fecal oocysts in all the drug-treated mice was significantly decreased compared with the positive control group (P <0.05). The histopathology and ultrastructure of small intestinal mucosa showed that the small intestine epithelium was in repair, And the combination therapy group was significantly better than the single-agent treatment group (P <0.05). Conclusion Chinese medicine psoralen and dihydroartemisinin have inhibitory effects on Cryptosporidium, but the latter insecticidal effect is obvious. The two act synergistically to participate in the host’s immune regulation and inflammatory repair processes.