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通过对休眠(D_0)、层积(格St)、埋藏(A_0)及散落(D_1)的水曲柳种子抑制物的生测及GC检测的比较,阐明了枯落物下埋藏水曲柳种子有利于其天然更新的实质。TLC(薄层层析)及生测结果表明,水曲柳种子的抑制物水平与休眠程度存在明显相关,其抑制物主要分布在β—抑制剂及高Rf值、低Rf值三区。休眠种子抑制水平最高,其外种皮的抑制活性超过种仁。经层积及埋藏,各区段抑制活性均明显下降,尤其是外种皮。但对根长抑制的缓解均滞后于发芽。A_0抑制活性介于D_0及St之间,表明埋藏是有利于后熟及更新的。散落种子抑制物多被破坏,但种仁的抑制活性甚至超过D_0,表明散落逆境深化了休眠,是更新难的原因。对主要抑制区段存在的亚油酸及油酸的生测表明,它们均抑制萌发。对ABA的定量测定结果,存在着与生测基本吻合的D_0>A_0>St>D_1的顺序,表明ABA与休眠程度密切相关。根据对各区段甲酯化与否的GC测定,推测了内源抑制物的可能特点。
Through the comparison of dormancy (D_0), stratification (St St), burial (A_0) and scattered (D_1) seed manure inhibitor determination and GC detection, elucidated the litter buried Ash seed Conducive to the nature of its natural renewal. The results of TLC and bioassay showed that there was a significant correlation between the level of inhibitor and the dormancy level of the seeds, and the inhibitors mainly distributed in the three regions of β-inhibitor, high Rf value and low Rf value. Hypocotyls inhibited the highest level of seed, and the inhibitory activity of the seed coat was higher than that of seed kernels. After stratification and burial, the inhibitory activity of each section decreased significantly, especially the outer seed coat. However, the suppression of root length lags behind germination. The inhibitory activity of A_0 was between D_0 and St, indicating that the burial is favorable for ripening and regeneration. The scattered seed inhibitors were mostly destroyed, but the inhibitory activity of seed kernel even exceeded D 0, indicating that the scattered adversity deepened dormancy and was the reason for the renewal. Bioassays of linoleic acid and oleic acid, which present the major inhibitory sections, showed that they all inhibited germination. The results of quantitative determination of ABA, there is basically consistent with the biochemical test D_0> A_0> St> D_1 order, indicating that the degree of ABA and sleep are closely related. Based on the GC determination of methyl esterification in each section, the possible characteristics of endogenous inhibitors were speculated.