论文部分内容阅读
1.本实驗用豚鼠15只,在其背部皮肤一定部位以65—67℃之热水烧伤器历3分钟制成烧伤。經不同时間(1—13天)取材,观察皮肤愈合期間糖元,透明貭酸,过碘酸雪夫氏反应(PAS)阳性物貭,核糖核酸(RNA)及碱性磷酸酶(ALP)之改变。同时对一般形态变化也作了观察。 2.烧伤后,伤口边緣表皮增厚,前沿出現迁移的薄层表皮細胞,浸在周围的渗出液內。其中未見絲状分裂,而經常为血液細胞浸潤。此类血細胞来自坏死的皮肌下的皮下組織,同处也是肉芽組織发生地。真皮不参加肉芽組織的形成。真皮为新生表皮被复时,其胶原纤維为异物巨細胞所包围。 3.糖元在伤区周緣表皮的生发层內和在迁移表皮的各层。自迁移表皮至伤区周緣增厚表皮糖元逐漸消失,首先在基层,次在棘层,最后完全消失。表皮角貭层近粒层的二,三层细胞含有抗消化的PAS阳性弥散顆粒。同样較細的顆粒也存在于未角化的新生表皮的表层細胞內。透明貭酸存在新生的肉芽組織內。 4.伤口周緣表皮內RNA成梯度分布,基层最富。此种梯度在迁移表皮內逐漸消失。透明角貭顆粒为RNA強阳性,仅次核仁含量,近核处此种顆粒成粗大团块状,紧与核膜相連,但在胞貭周緣則顆粒变細。 5.RNA在毛根外鞘,毛髓貭,和新生表皮內的分布和糖元成相反的关系。 6.ALP在白血球浸潤处,和渗出液,呈阳性反应。 7.糖元,透明角貭顆粒,抗消化PAS阳性物貭和渗出液的存在意义进行了討論。
1. In this experiment, 15 guinea pigs were used, and burns were made in hot water of 65-67 ℃ for 3 minutes on certain parts of their back skin. At different times (1-13 days), the contents of glycogen, hyaluronic acid, PAS-positive substance, RNA and ALP during skin healing were observed. change. At the same time, general morphological changes were also observed. 2. After burns, the wound edge of the epidermis thickening, the emergence of migrating thin layer of epidermal cells, immersed in the surrounding exudate. No filamentous fissures were found therein, but often infiltrated by blood cells. Such blood cells from the skin under the necrotic subcutaneous tissue, is also the place with granulation tissue. Dermis does not participate in the formation of granulation tissue. The dermis is complex when newborn epidermis, the collagen fiber is surrounded by foreign body giant cells. 3. Glycogen in the injured area of the epidermal germinal layer and in the migration of epidermal layers. Since the migration of the epidermis to the peripheral edge of the wounded area of epidermal glycogen gradually disappear, first in the grass-roots, secondary in the spinous layer, and finally disappeared. The second and third layers of the parietal layer of the cuticle of the cuticle contain anti-digestive PAS-positive diffuse granules. The same finer particles are also present in the surface cells of unincorporated nascent epidermis. Transparent behenic acid is present in nascent granulation tissue. 4. Wound peri-epidermal RNA gradient distribution, the richest grass-roots. This gradient disappears within the migrating epidermis. Transparent angle 貭 particles strongly positive for RNA, only the second kernel content, near the nucleus of such particles into a large mass, closely linked with the nuclear membrane, but in the perineum around the particles become thinner. The distribution of RNA in the hairy sheaths, vellus, and nascent epidermis is inversely related to glycogen. 6. ALP leukocyte infiltration, and exudate, was positive. 7. Glycogen, transparent angle 貭 particles, the anti-digestive PAS positive substance 貭 and the significance of the exudate were discussed.