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目的:研究次声暴露对离体及在体小鼠结肠癌CT26细胞生长的影响。方法:离体实验将小鼠结肠癌CT26细胞接种后分为对照组、8Hz/130dB组和16Hz/130dB组;在体实验将小鼠结肠癌CT26细胞接种于BALB/c小鼠皮下建立动物模型后,分为对照组、8Hz/100dB组和16Hz/100dB组。每天均暴露2h,连续7d。离体细胞每天干预后采用MTT法检测细胞增殖活性;动物模型于暴露第7天摘除小鼠眼球留取血液标本,并处死后留取肿瘤组织,观察移植瘤大体形态和组织病理学改变,计算抑瘤率;免疫组化法测定凋亡相关蛋白Caspase-3的表达情况;酶联免疫法测定血清癌胚抗原(CEA)水平。结果:1)离体8Hz/130dB组及16Hz/130dB组与对照组相比结肠癌CT26细胞增殖活性在暴露d3～d6时均明显降低,P<0.05。2)在体8Hz/100dB组、16Hz/100dB组小鼠结肠癌移植瘤出现坏死性病理变化。3)在体Caspase-3表达阳性着色于细胞核呈褐色,16Hz/100dB组的Caspase-3表达明显高于对照组,P<0.05;16Hz/100dB组血清CEA水平(1 901.92±362.82)ρg/mL较对照组明显降低(3 929.14±2 934.33)ρg/mL,P=0.041。结论:8Hz/130dB、16Hz/130dB次声暴露可抑制离体结肠癌CT26细胞的增殖;16Hz/100dB可致在体小鼠结肠癌移植瘤坏死;16Hz/100dB可促进在体结肠癌CT26细胞的凋亡,降低小鼠体内血清CEA水平。
Objective: To study the effect of infrasound on the growth of colon carcinoma CT26 cells in vitro and in vivo. Methods: Colon cancer CT26 cells were inoculated into control group, 8Hz / 130dB group and 16Hz / 130dB group in vitro. CT26 cells were inoculated subcutaneously into BALB / c mice to establish animal models After divided into control group, 8Hz / 100dB group and 16Hz / 100dB group. Daily exposure 2h, continuous 7d. The cell proliferation was measured by MTT method after the intervention in vitro. On the 7th day after exposure, the blood samples were removed from the eyeballs of mice and the tumor tissues were removed after sacrifice. The morphological and histopathological changes of the tumors were observed and calculated The tumor inhibition rate was determined by immunohistochemistry. The expression of Caspase-3 was detected by immunohistochemistry. The level of serum carcinoembryonic antigen (CEA) was determined by enzyme-linked immunosorbent assay. Results: 1) Proliferation of colon cancer CT26 cells in 8Hz / 130dB group and 16Hz / 130dB group were significantly decreased at d3 ~ d6, P <0.05.2) / 100dB group of colon cancer xenograft tumor pathological changes. 3) The positive staining of Caspase-3 in the nucleus was brown, the expression of Caspase-3 in 16Hz / 100dB group was significantly higher than that in the control group, P <0.05; CEA level in 16Hz / 100dB group was 1 901.92 ± 362.82 ρg / mL Which was significantly lower than that of the control group (3929.14 ± 2934.33) ρg / mL, P = 0.041. Conclusion: Infrasound of 8Hz / 130dB and 16Hz / 130dB can inhibit the proliferation of colon cancer CT26 cells in vitro. 16Hz / 100dB can induce necrosis of colon carcinoma in vivo in mice and 16Hz / 100dB can promote CT26 cells in colon cancer Apoptosis, reduce serum CEA levels in mice.