论文部分内容阅读
揭示干旱条件下植物耐高温的能力,可提高品种抗干旱的潜力。在土壤水分为8%~9%干旱条件下,分别在28和48℃处理耐旱的T7代过表达Gm HSFA1大豆株系,观察其形态基因表达和生理及光合指标的变化,并运用关联分析方法,筛选和确定抗干旱耐高温的大豆品系,为大豆分子遗传改良和基因聚合育种的种质材料利用奠定技术基础。结果表明:在干旱、高温条件下,过表达Gm HSFA1大豆株系目的基因表达量明显增高,其中T7-27大豆的表达量增加了22倍;植株中热激蛋白的靶基因HSP70、HSP22、HSP17.9的表达量明显上调,分别增加了46,7和59倍;脯氨酸含量明显增加;丙二醛含量受高温影响,株系间增幅不同;可溶性糖含量受高温影响均明显增加,增幅最高的为T7-27株系,增幅为91%;植株净光合速率降低,但低于非转基因大豆。多种指标的灰色关联性分析表明,过表达Gm HSFA1的大豆株系T7-18和T7-27的抗干旱耐热性较好。
To reveal the ability of plants to withstand high temperatures under drought conditions can improve the drought resistance potential of the cultivars. Under drought conditions with soil moisture of 8% ~ 9%, drought-resistant T7 generation overexpressing Gm HSFA1 soybean lines were treated at 28 and 48 ℃, respectively. The morphological, physiological and photosynthetic characteristics were observed. Correlation analysis Method to screen and identify the drought-resistant and high-temperature-tolerant soybean lines, which laid the technical foundation for the utilization of the germplasm materials for soybean molecular genetic improvement and gene polymerization breeding. The results showed that under drought and high temperature, the gene expression of Gm HSFA1 overexpressing soybean lines was significantly increased, of which the expression of T7-27 soybean increased by 22 times; the heat shock protein target genes HSP70, HSP22, HSP17 .9 expression increased significantly, respectively, increased by 46,7 and 59 times; proline content increased significantly; MDA content affected by high temperature, different strains increased; soluble sugar content by the high temperature were significantly increased, the increase The highest was T7-27, an increase of 91%. The net photosynthetic rate of plants decreased but was lower than that of non-GMO soybean. Gray relational analysis of multiple indicators indicated that the soybean lines T7-18 and T7-27 overexpressing Gm HSFA1 had better drought resistance and heat tolerance.