Objective:This study is to investigate the medicinal value of methanolic extiact of the leaves of Musa sapientum var.sylvesteris in Bangladesh.Methods:Several biochemical assays,thin layer chormatogarphy and ultra-violet spectroscopy were used to detect the presence of various types of compounds in this extract.Antioxidant effects were measured by DPPH scavenging assay,total reducing assay and hydrogen peroxide scavenging assay.Receptor binding activities and hydrogen peroxide induced hemolysis assay were performed by hemagglutination assay and hemolysis assay using erythrocytes.Disk diffusion assay was performed to show the antibacterial effect of the extract.Results:Alethanolic extract of the leaves showed antioxidant and antibacterial activity in ritro.The extract showed hemaglutination inhibition activities and hydrogen peroxide induced hemolysis inhibition activity of human red blood cells.Conclusion:Musa sapientum var.sylvesteris can be an useful medicinal plant. Objective: This study is to investigate the medicinal value of methanolic extiact of the leaves of Musa sapientum var.sylvesteris in Bangladesh. Methods: Several Biochemical assays, thin layer chormatogarphy and ultra-violet spectroscopy were used to detect the presence of various types of compounds in this extract. Antioxidant effects were measured by DPPH scavenging assay, total reducing assay and hydrogen peroxide scavenging assay. Receptor binding activities and hydrogen peroxide induced hemolysis assay were performed by hemagglutination assay and hemolysis assay using erythrocytes. Disk diffusion assay was performed to show the antibacterial effect of the extract. Results: Alethanolic extract of the leaves showed antioxidant and antibacterial activity in ritro. The extract showed hemaglutination inhibition activities and hydrogen peroxide induced hemolysis inhibition activity of human red blood cells. Conlusion: Musa sapientum var.sylvesteris can be an useful medicinal plant.