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目的:观察银杏叶提取物(EGb)对新生大鼠缺氧缺血性脑损伤(HIBD)后脑内基质金属蛋白酶表达的影响。方法:7日龄SD幼鼠32只随机分为正常对照组(ZC组)、假手术组(SH组)、银杏叶提取物干预组(EGb组)、生理盐水对照组(NS组),采用经典的Rice法制作HIBD模型,ZC组不做任何处理,EGb组模型建立后即可腹腔注射银杏内酯B 10 mg/kg,每日1次,连续注射10天,NS组每日腹腔注射生理盐水共10天,采用免疫组化方法观察大脑皮质MMP-2、MMP-9的分布及表达。结果:NS组与ZC组相比,MMP-2、MMP-9在大鼠脑皮质层表达明显增加(P<0.01),MMP-2在第4天表达最为明显,MMP-9在24 h时表达最为明显;EGb组与NS组各个时间段相比,MMP-2、MMP-9表达明显减少(P<0.01)。结论:EGb可减少新生大鼠缺氧缺血性脑损伤后的MMP-2、MMP-9的表达,对缺氧缺血性脑损伤新生大鼠脑有神经保护作用。
Objective: To observe the effects of Ginkgo biloba extract (EGb) on the expression of matrix metalloproteinases in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods: Thirty-two SD rats of 7 days old were randomly divided into normal control group (ZC group), sham operation group (SH group), Ginkgo biloba extract intervention group (EGb group) and saline control group (NS group) The classic method of Rice HIBD model, ZC group without any treatment, EGb group model can be injected intraperitoneally ginkgolide B 10 mg / kg, once daily for 10 days, NS group daily intraperitoneal injection of physiology Saline for 10 days. Immunohistochemistry was used to observe the distribution and expression of MMP-2 and MMP-9 in the cerebral cortex. Results: Compared with ZC group, the expression of MMP-2 and MMP-9 in NS group was significantly increased (P <0.01), the expression of MMP-2 was most obvious on the 4th day, the expression of MMP- The expression of MMP-2 and MMP-9 in EGb group was significantly lower than that in NS group (P <0.01). Conclusion: EGb can reduce the expression of MMP-2 and MMP-9 after hypoxic-ischemic brain damage in neonatal rats, and protect the brain of neonatal rats with hypoxic-ischemic brain damage.