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目的设计一种基于SiO_2荧光纳米粒子快速检测果汁中大肠杆菌O157:H7的新型传感器。方法采用超分子组合法将荧光基团嵌入到SiO_2纳米颗粒中合成SiO_2荧光纳米粒子,然后将抗大肠杆菌O157:H7的单抗偶联到纳米粒子表面,最后通过抗原-抗体反应使纳米粒子与待检细菌结合,使用荧光显微镜观察并统计大肠杆菌O157:H7的个数。结果本方法与革兰氏染色法相比,对大肠杆菌O157:H7计数结果无统计学差异(P=0.930);该方法整个检测过程能够在15 min内完成,检测下限小于10 CFU/mL。结论该方法能增强检测果汁中大肠杆菌O157:H7的灵敏度,缩短检测时间,对其他病原体检测方法的改进具有重要的指导意义。
Objective To design a novel sensor based on SiO_2 fluorescent nanoparticles for rapid detection of Escherichia coli O157: H7 in fruit juice. Methods Fluorescent groups were embedded in SiO_2 nanoparticles to synthesize SiO_2 fluorescent nanoparticles by superamolecular assembly method. Then the anti-E.coli O157: H7 monoclonal antibody was coupled to the surface of the nanoparticles. Finally, the nanoparticles were reacted with Bacteria to be tested for binding, using a fluorescence microscope to observe and count the number of E. coli O157: H7. Results Compared with Gram stain method, there was no significant difference (P = 0.930) in the counting results of Escherichia coli O157: H7. The whole detection could be completed in 15 minutes and the detection limit was less than 10 CFU / mL. Conclusion The method can enhance the sensitivity of E. coli O157: H7 in fruit juice and shorten the test time, which is of great guiding significance for the improvement of other pathogens detection methods.